Emodin Exhibits Strong Cytotoxic Effect in Cervical Cancer Cells by Activating Intrinsic Pathway of Apoptosis

Pharmacognosy Research,2022,14,4,499-503.DOI:10.5530/pres.14.4.72Published:October 2022Type:Original ArticleAuthors:Amir Saeed, and Ahmed Alharbi Author(s) affiliations:Amir Saeed1,2, Alharbi1 1Department of Medical Laboratory Sciences, College Applied University Hail, KSA. 2Department Microbiology, Faculty Sciences Technology, Khartoum, SUDAN. Abstract:Background: Cervical cancer is widely acknowledged as the main cause mortality a major obstacle to increasing life expectancy, after breast cancer, in women. Natural products have been proven be promising source for development potential anticancer drugs. Emodin (1,3,8-trihydroxy-6-methyl-9,10-anthracenedione) shown exert wide range biological effects, including antibacterial, hepatoprotective, anti-inflammatory anticancer. Aim: The aim this study was evaluate efficacy emodin against progression human cervical cancer. Materials Methods: cytotoxic effect on HeLa cells assessed by cell viability assay phase contrast microscopy. Moreover, DAPI staining performed analyze nuclear condensation. H2DCFDA done ROS generation. Activation caspases determined ascertain mitochondria-mediated apoptosis cells. Results: results revealed that strongly inhibited growth proliferation; which validated authenticated condensation fragmentation indicating initiation apoptosis. We also observed significant generation activation emodin-treated Conclusion: Our suggested strong Keywords:Apoptosis, Cancer, DAPI, Emodin, HeLa, ROSView:PDF (807.5 KB)